Calpain

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Calpains (EC 3.4.22.52, EC 3.4.22.53) are a family of calcium-dependent, non-lysosomal cysteine proteases (proteolytic enzymes) expressed ubiquitously in mammals and many lower organisms. Calpains constitute the C2 family of protease clan CA in the MEROPS database. The calpain proteolytic system includes the calpain proteases, the small regulatory subunit (calpain 4), and the endogenous calpain-specific inhibitor, calpastatin.

Contents 1 Historical background 2 Physiological roles 3 Calpains in pathologies 4 Protein structure 5 Cleavage specificity 6 References 7 External links

[edit] Historical background

The history of calpain originates during the mid-1960s, when calcium-dependent proteolytic activities caused by a “calcium-activated neutral protease” (CAPN) were detected in brain, lens of the eye and other tissues. In the late 1960s the enzymes were isolated and characterised independently in both rat brain and skeletal muscle. These activities were caused by an intracellular cysteine protease not associated with the lysosome and having an optimum activity at neutral pH, which clearly distinguished it from the cathepsin family of proteases. The calcium-dependent activity, intracellular localization, along with the limited, specific proteolysis on its substrates, highlighted calpain’s role as a regulatory, rather than a digestive protease. When the sequence of this enzyme became known, it was given the name “calpain”, to recognize it as a hybrid of two well-known proteins at the time, the calcium-regulated signaling protein, calmodulin, and the cysteine protease of papaya, papain. Shortly thereafter, the activity was found to be attributable to two main isoforms, dubbed μ("mu")-calpain and m-calpain (a.k.a. calpain 1 and 2), that differed primarily in their calcium requirements in vitro. Their names reflect the fact that they are activated by micro- and millimolar concentrations of Ca²⁺ within the cell, respectively.^[1] To date, these two isoforms remain the best characterised members of the calpain family. Structurally, these two heterodimeric isoforms share an identical small (30k) subunit (calpain 4), but have distinct large (80k) subunits.

Recently, sequencing of the human genome has revealed genes for more than a dozen other calpain isoforms, some with multiple splice variants. As the first calpain whose almost complete three-dimensional structure was determined, m-calpain is the type-protease for the C2 (calpain) family in the MEROPS database.

[edit] Physiological roles

Although the physiological roles of calpains are still poorly understood, they have been shown to be active participants in processes such as cell mobility and cell cycle progression, as well as cell-type specific functions such as long-term potentiation in neurons and cell fusion in myoblasts. Under these physiological conditions, a transient and localized influx of calcium into the cell activates a small local population of calpains (for example, those close to Ca²⁺ channels), which then advance the signal transduction pathway by catalyzing the controlled proteolysis of its target proteins. Other reported roles of calpains are in cell function, helping to regulate clotting and the diameter of blood vessels, and playing a role in memory. Calpains have been implicated in apoptotic cell death, and appear to be an essential component of necrosis.

In the brain, while μ-calpain is mainly located in the cell body and dendrites of neurons and to a lesser extent in axons and glial cells, m-calpain is found in glia and a small amount in axons.^[2] Calpain is also involved in skeletal muscle protein breakdown due to exercise and altered nutritional states.^[3]

[edit] Calpains in pathologies

The structural and functional diversity of calpains in the cell is reflected in their involvement in the pathogenesis of a wide range of disorders. At least two well known genetic disorders and one form of cancer have been linked to tissue-specific calpains. When defective, the mammalian calpain 3 (also known as p94) is the gene product responsible for limb-girdle muscular dystrophy type 2A, calpain 10 has been identified as a susceptibility gene for type II diabetes mellitus, and calpain 9 has been identified as a tumor suppressor for gastric cancer. Moreover, the hyperactivation of calpains is implicated in a number of pathologies associated with altered calcium homeostasis such as Alzheimer’s disease, and cataract formation, as well as secondary degeneration resulting from acute cellular stress following myocardial ischemia, cerebral (neuronal) ischemia, traumatic brain injury and spinal cord injury. Excessive amounts of calpain can be activated due to Ca²⁺ influx after cerebrovascular accident (during the ischemic cascade) or some types of traumatic brain injury such as diffuse axonal injury). The sustained influx of calcium into the cell results in calpain hyperactivation, unregulated proteolysis of both target and non-target proteins and consequent irreversible tissue damage. Excessively active calpain breaks down molecules in the cytoskeleton such as spectrin, microtubule subunits, microtubule-associated proteins, and neurofilaments.^[4] It may also damage ion channels, other enzymes, cell adhesion molecules, and cell surface receptors.^[2] This can lead to degradation of the cytoskeleton and plasma membrane. Calpain may also break down sodium channels that have been damaged due to axonal stretch injury,^[5] leading to an influx of sodium into the cell. This, in turn, leads to the neuron's depolarization and the influx of more Ca²⁺. The exogenous regulation of calpain activity is therefore of interest for the development of therapeutics in a wide array of pathological states. As a few of the many examples supporting the therapeutic potential of calpain inhibition in ischemia, calpain inhibitor AK275 protected against focal ischemic brain damage in rats when administered after ischemia, and MDL28170 significantly reduced the size of damaged infarct tissue in a rat focal ischemia model.

In addition to damaging cell structures, calpain indirectly creates free radicals. The protease cleaves the enzyme Xanthine Dehydrogenase to Xanthine oxidase (XOA) which, in turn, creates superoxide radicals, oxygen free radicals composed of two oxygen atoms with an extra, unpaired electron.^[6]

Calpain may be released in the brain for up to a month after a head injury, and may be responsible for a shrinkage of the brain sometimes found after such injuries.^[7] However, calpain may also be involved in a "resculpting" process that helps repair damage after injury.^[7]

[edit] Protein structure

Calpain 1 and 2 are heterodimeric enzymes that share a common 21k regulatory subunit (encoded by the calpain 4 gene).

[edit] Cleavage specificity

No specific amino acid sequence is uniquely recognized by calpains. Amongst protein substrates, tertiary structure elements rather than primary amino acid sequences are likely responsible for directing cleavage to a specific substrate. Amongst peptide and small-molecule substrates, the most consistently reported specificity is for small, hydrophobic amino acids (e.g. leucine, valine and isoleucine) at the P2 position, and large hydrophobic amino acids (e.g. phenylalanine and tyrosine) at the P1 position.^[8] Arguably, the best currently available fluorogenic calpain substrate is (EDANS)-Glu-Pro-Leu-Phe=Ala-Glu-Arg-Lys-(DABCYL), with cleavage occurring at the Phe=Ala bond.

[edit] References

1. ^ Glass JD, Culver DG, Levey AI, and Nash NR. (2002). Very early activation of m-calpain in peripheral nerve during Wallerian degeneration. Journal of the Neurological Sciences. 196(1-2): 9-20.
2. ^ ^{a b} Lenzlinger PM, Saatman KE, Raghupathi R, Mcintosh TK. 2001. Overview of basic mechanisms underlying neuropathological consequences of head trauma. Chapter 1. In, Head trauma: Basic, preclinical, and clinical directions. Miller LP and Hayes RL, eds. Wiley Liss, (a John Wiley and Sons publication) New York.
3. ^ Belcastro AN, Albisser TA, Littlejohn B (1996). Role of calcium-activated neutral protease (calpain) with diet and exercise. "Canadian Journal of Applied Physiology". 21(5):328-346. PMID 8905185. Retrieved January 19, 2007.
4. ^ Castillo MR and Babson JR. (1998). Ca²⁺-dependent mechanisms of cell injury in cultured cortical neurons. Neuroscience. 86(4): 1133-1144. PMID 9697120 Retrieved on January 19, 2007.
5. ^ Iwata A, Stys PK, Wolf JA, Chen XH, Taylor AG, Meaney DF, and Smith DH. (2004). Traumatic Axonal Injury Induces Proteolytic Cleavage of the Voltage-Gated Sodium Channels Modulated by Tetrodotoxin and Protease Inhibitors. The Journal of Neuroscience. 24(19): 4605-4613. PMID 15140932 Retrieved on January 19, 2007.
6. ^ Fouad T. (2003). Free Radicals, Types, Sources and Damaging Reactions. The Doctors Lounge. Retrieved on January 19, 2007.
7. ^ ^{a b} White V. (1999). 'Biochemical Storm' Following Brain Trauma an Important Factor in Treatment, University of Florida Researcher Finds. Retrieved on January 19, 2007.
8. ^ Cuerrier D, et al (2005). Determination of peptide substrate specificity for mu-calpain by a peptide library-based approach: the importance of primed side interactions. Journal of Biological Chemistry. 280(49):40632-40641. PMID 16216885 Retrieved on January 19, 2007.

• Büki A, Okonkwo D. O., Wang K. K. W., and Povlishock J. T. (2000). Cytochrome c Release and Caspase Activation in Traumatic Axonal Injury. The Journal of Neuroscience. 20(8): 2825-2834. Retrieved on January 19, 2007.
• Karp G. (2005). Cell and Molecular Biology: Concepts and Experiments, Fourth ed, pp. 148, 165-170, and 624-664. John Wiley and Sons, Hoboken, NJ.

[edit] External links

• The Calpain Family of Proteases. (2001). University of Arizona.
• Wang, K.K.W. and Yuen, P.-w. (Eds.) (1999) Calpain: The Pharmacology and Toxicology of Calcium-Dependent Protease. Taylor and Francis, PA; 22 Chapters, 441 pages.
• MeSH Calpain