Bimolecular Fluorescence Complementation
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Bimolecular Fluorescence Complementation (BiFC) is a means of observing protein-protein interactions. Two proteins of interest are tagged with one half of a fluorescent protein. If the two proteins come together and the tags unite then the full fluorescent protein can form. However, this process can take 60 seconds, a limiting factor for applications of this approach.
BiFC can be used as an alternative to FRET, or it can complement it.
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Hu, CD, Grinberg, AV and Kerppola, TK. Visualization of protein interactions in living cells using bimolecular fluorescence complementation (BiFC) analysis. Current Protocols in Cell Biology, 21.3. Wiley Interscience, 2005
