Bicinchoninic acid
From Wikipedia, the free encyclopedia
Bicinchoninic acid | |
---|---|
General | |
Systematic name | 2-(4-carboxyquinolin-2-yl)quinoline-4-carboxylic acid |
Other names | Bicinchoninic acid 4,4'-Dicarboxy-2,2'-Biquinoline |
Molecular formula | (HO2CC9H5N)2 |
Molar mass | 344.33 g/mol |
Appearance | Cream powder. Characteristic odor. |
CAS number | [1245-13-2] |
Properties | |
Density and phase | Solid in pure form. Density not available. |
Solubility in water | Partially soluble in cold water, hot water. |
Other solvents | Soluble in alcohol and aqueous alkali. |
Melting point | 365-367°C |
Boiling point | Not available. |
Acidity (pKa) | ? |
Structure | |
Crystal structure | ? |
Dipole moment | ? D |
Hazards | |
MSDS | External MSDS |
Main hazards | Will irritate eyes and mucous membranes. The chemical, physical and toxicological properties have not been fully investigated. Presume moderate toxicity internally. LD/TD no data. OSHA PEL/ACGIH TLV not established. No evidence of carcinogenicity. |
NFPA 704 | |
Flash point | N/A |
RTECS number | N/A |
Except where noted otherwise, data are given for materials in their standard state (at 25°C, 100 kPa) Infobox disclaimer and references |
Bicinchoninic acid is a weak acid composed of two carboxylated quinoline rings.
Bicinchoninic acid is most commonly employed by biochemists in the bicinchoninic acid assay, which is used to determine the total level of protein in a solution. In this assay, two molecules of bicinchoninic acid chelate a single Cu1+ ion, forming a purple water-soluble complex that strongly aborbs light at 562 nm.