Ancestral reconstruction

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Originally proposed by Pauling and Zuckerkandl in 1963^[1] the reconstruction of ancient proteins and DNA sequences has only recently become a significant scientific endeavor. The developments of extensive genomic sequence databases in conjunction with advances in biotechnology and phylogenetic inference methods have made ancestral reconstruction cheap, fast, and scientifically practical.

Ancestral protein and DNA reconstruction allows for the recreation of protein and DNA evolution in the laboratory so that it can be studied directly^[2]. With respect to proteins, this allows for the investigation of the evolution of present-day molecular structure and function. Additionally, ancestral protein reconstruction can lead to the discoveries of new biochemical functions that have been lost in modern proteins^[3]. It also allows insights into the biology and ecology of extinct organisms^[4]. Although the majority of ancestral reconstructions have dealt with proteins, it has also been used to test evolutionary mechanisms at the level of bacterial genomes^[5] and primate gene sequences^[6].

In summary, ancestral reconstruction allows for the study of evolutionary pathways, adaptive selection, and functional divergence of the evolutionary past. For a review of biological and computational techniques of ancestral reconstruction see Chang et al^[7]. For criticism of ancestral reconstruction computation methods see Williams P.D. et al^[8].

[edit] Notes and references

^ Pauling L. and Zuckerkandl E. (1963). "Chemical paleogenetics, molecular restoration studies of extinct forms of life". Acta chemica Scandinavica 17 (89): 9-16.
^ Chang S.W.; Ugalde J.A.; Matz M.V. (2005). "Applications of Ancestral Protein Reconstruction in Understanding Protein Function: GFP-Like Proteins". Methods in Enzymology 395: 652-670.
^ Jermann T. M. et al (1995). "Reconstructing the evolutionary history of the artiodactyl ribonuclease superfamily". Nature 374 (6517): 57-59.
^ Chang B.S. et al (2002). "Recreating a functional ancestral archosaur visual pigment". Molecular Biology and Evolution 19 (9): 1483-1489.
^ Zhang C. et al (2003). "Genome diversification in phylogenetic lineages I and II of Listeria monocytogenes: identification of segments unique to lineage II populations". Journal of Bacteriology 185 (18): 5573-5584.
^ Krishnan N.M. et al (2004). "Ancestral sequence reconstruction in primate mitochondrial DNA: compositional bias and effect on functional inference". Molecular Biology and Evolution 21 (10): 1871-1883.
^ Chang S.W.; Ugalde J.A.; Matz M.V. (2005). "Applications of Ancestral Protein Reconstruction in Understanding Protein Function: GFP-Like Proteins". Methods in Enzymology 395: 652-670.
^ Williams P.D. et al (2006). "Assessing the accuracy of ancestral protein reconstruction methods". PLoS Computational Biology 2 (6): e69.