Amplified fragment length polymorphism
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Amplified fragment length polymorphism PCR, or "AFLP-PCR" (often AFLP), is a tool used in the study of genetics and in the practice of genetic engineering.
AFLP-PCR is a highly sensitive method for detecting polymorphisms in DNA.The technique was originally described by Vos and Zabeau in 1993. The procedure of this technique is divided into three steps:
- Digestion of total cellular DNA with one or more restriction enzymes and ligation of restriction half-site specific adaptors to all restriction fragments.
- Selective amplification of some of these fragments with two PCR primers that have corresponding adaptor and restriction site specific sequences.
- Electrophoretic separation of amplicons on a gel matrix, followed by visualisation of the band pattern.
A variation on AFLP is TE Display, used to detect transposable element mobility.
[edit] See also
[edit] References
- Zabeau, M and P. Vos. 1993. Selective restriction fragment amplification: a general method for DNA fingerprinting. European Patent Office, publication 0 534 858 A1, bulletin 93/13.
- Vos, P., Hogers, R., Bleeker, M., et al. 1995. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research 23(21):4407-4414 [1]
- Van den Broeck et al., 1998
- Casa et al., 2000
- Biedler et al., 2003
[edit] External links
Online programs for simulation of AFLP-PCR
- ALFIE - BProkaryotes or uploaded sequences
- In silico AFLP-PCR for prokaryotes,some eukaryotes or uploaded sequences
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