Aldolase A

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Aldolase A
Enzyme Fructose 1,6-bisphosphate aldolase PDB Code PDB 4ALD Organism Human Tissue Muscle Complexed molecules fructose 1,6-bisphosphate

Aldolase A is an enzyme which catalyses one of the aldol reactions: The substrate, fructose 1,6-bisphosphate (F-1,6-BP) is broken down into glyceraldehyde 3-phosphate and dihydroxyacetone phosphate (DHAP). This reaction is a part of glycolysis.

β-D-fructose 1,6-phosphate	fructose bisphosphate aldolase		D-glyceraldehyde 3-phosphate		dihydroxyacetone phosphate
				+
	fructose bisphosphate aldolase

Compound C05378 at KEGG Pathway Database. Enzyme 4.1.2.13 at KEGG Pathway Database. Compound C00111 at KEGG Pathway Database. Compound C00118 at KEGG Pathway Database.

The numbering of the carbon atoms indicates the fate of the carbons according to their position in fructose 6-phosphate.

[edit] Mechanism

In mammalian aldolase the key catylitic amino acid residues involved in the reaction are lysine and tyrosine. The tyrosine acts as an efficient hydrogen acceptor while the lysine covalently binds and stabilizes the intermediates. Many bacteria use two magnesium ions in place of the lysine.

The reaction mechanism of aldolase.
The enzyme's reactive site amino acid's side chains are shown in blue.

Abbreviations: DHAP - dihydroxyacetone phosphate; Fru1,6bP - Fructose-1,6-bisphosphate; GAD - glyceraldehyde 3-phosphate;

[edit] References

• http://pdbdev.sdsc.edu:48346/pdb/molecules/pdb50_5.html
• MeSH Aldolases

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v • d • e  Glycolysis Metabolic Pathway

Glucose Glucose-6-phosphate Fructose 6-phosphate Fructose 1,6-bisphosphate Dihydroxyacetone phosphate Glyceraldehyde 3-phosphate Glyceraldehyde 3-phosphate ATP ADP ATP ADP NAD+ + Pi NADH + H+ + 2 NAD+ + Pi NADH + H+ 1,3-Bisphosphoglycerate 3-Phosphoglycerate 2-Phosphoglycerate Phosphoenolpyruvate Pyruvate Acetyl-CoA ADP ATP H2O ADP ATP CoA + NAD+ NADH + H+ + CO2 2 2 2 2 2 2 ADP ATP H2O