ABTS

From Wikipedia, the free encyclopedia

ABTS
IUPAC name	2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid)
Properties
Molecular formula	C18H18N4O6S4
Molar mass	514.62 g/mol
Hazards
R-phrases	Template:36/37/38
S-phrases	S26-S36
Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) Infobox disclaimer and references

In biochemistry, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) or ABTS is chemical compound used to observe the reaction kinetics of specific enzymes.

It is commonly used as a co-substrate with hydrogen peroxide for a peroxidase enzyme or alone with a laccase enzyme. Its use allows the reaction kinetics of peroxidases themselves to be followed. In this way it also can be used to indirectly follow the reaction kinetics of any hydrogen peroxide producing enzyme, or to simply quantify the amount of hydrogen peroxide in a sample. The reason this compound is chosen, is because it is converted by the enzyme into a green and soluble end-product, and thus it's absorbance of 405 nm light can easily be followed with a spectrophotometer, a common laboratory instrument. It is sometimes used as part of a glucose estimating reagent in finding glucose concentrations of solutions such as blood serum.