3,3’,5,5’-Tetramethylbenzidine
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| 3,3’,5,5’-Tetramethylbenzidine | |
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| Systematic name | 3,3',5,5'-tetramethylbiphenyl-4,4'-diamine |
| Chemical formula | C16H20N2 |
| Molecular mass | 240.3482 g/mol |
| Density | ? g/cm3 |
| Melting point | 192–196 °C |
| Boiling point | ? °C |
| CAS number | [54827-17-7] |
| SMILES | NC1=C(C)C=C(C2=CC(C)=C(N)C(C)=C2)C=C1C |
| Disclaimer and references | |
3,3’,5,5’-Tetramethylbenzidine or TMB is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA).
It is a white crystal powder which should be protected from exposure to sunlight, and a mutagen.
Forms a blue product when combined with a peroxidase enzyme; completion of the reaction results in a yellow coloration.
[edit] External links
- Safety (MSDS) data for 3,3',5,5'-tetramethylbenzidine
- Tetramethylbenzidine (TMB) - Ready to Use Chromogen
- Links to external chemical sources
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