Talk:Thin layer chromatography

From Wikipedia, the free encyclopedia

On what basis does tlc separate chemicals? Polarity? Charge? Size?... Aaadddaaammm 05:46, 3 September 2006 (UTC)

I changed the word "dye" to "compound," since "dye" usually refers to an organic compound. Also, it is not the spots themselves that fluoresce, but the Zinc Silicate added to the adsorbent. Mihovil 02:50, 19 September 2006 (UTC)

The TLC separation can be based on different techniques but the most common on a straight phase TLC plate is distribution (between stationary and mobile phase). There are several issues in the rest of the text too, i.e. correct is, that the fluorescence indicator does the fluorescing (can be caused by different fluorescing substances) of the layer but the spots (usually named 'fractions') on the plate can emit light if illuminated by a suitable wavelength or often have been derivatized to do so after chromatography (post chromatographic derivatization). To find more/accurate information about TLC (the modern version is called HPTLC = high performance TLC or Planar Chromatography) have a look at www.camag.com. If you want permission to copy from our website, please send us an email (info@camag.com) and then I can send you a nice picture of a TLC separation... PJ 27/Sep/06

[edit] Apropriate Solvant merger

Hi. I just merged the AS page with this one, as per request. If someone who has knowledge on this subject could cleanup my edits, I'd appreciate it.--C.lettinga 05:32, 15 December 2006 (UTC)