Sodium orthovanadate
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| Sodium orthovanadate | |
|---|---|
| General | |
| Systematic name | Sodium orthovanadate |
| Other names | sodium vanadate (V), sodium vanadium oxide |
| Molecular formula | Na3VO4 |
| Molar mass | 183.91 g/mol |
| Appearance | white powder |
| CAS number | [13721-39-6] |
| Properties | |
| Density and phase | 2.16 g/cm³, solid |
| Solubility in water | soluble |
| Melting range | 850-866°C |
| Hazards | |
| MSDS | External MSDS |
| Main hazards | Harmful. |
| NFPA 704 |
1
2
0
|
| Flash point | Non-flammable |
| RTECS number | YW1120000 |
| Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) Infobox disclaimer and references |
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Sodium orthovanadate is an inhibitor of protein tyrosine phosphatases, alkaline phosphatases and a number of ATPases, most likely acting as a phosphate analogue. The VO43- ion binds irreversibly to the active sites of most protein tyrosine phosphatases.
It is often added to buffer solutions that are used in protein analysis in molecular biology. The aim is to preserve the phosphorylation of proteins of interest by inhibiting endogenous phosphatases present in cell lysate mixture. It is commonly used at a final concentration of 10 - 100mM. It is toxic when inhaled, swallowed or in contact with skin.
[edit] References
- Swarup, G. et al. Biochem. Biophys. Res. Comm. 107: 1104-1109 (1982).
