Small hairpin RNA
From Wikipedia, the free encyclopedia
A short hairpin RNA (shRNA) is a sequence of RNA that makes a tight hairpin turn that can be used to silence gene expression.
shRNA is transcribed by RNA polymerase III. shRNA production in a mammalian cell can sometimes cause the cell to mount an interferon response as the cell seeks to defend itself from what it perceives as viral attack. This problem is not observed in miRNA, which is transcribed by RNA polymerase II (the same polymerase used to transcribe mRNA).
[edit] Bibliography
- Design and cloning strategies for constructing shRNA expression vectors
- RNA interference improves motor and neuropathological abnormalities in a Huntington's disease mouse model.
- Molecular strategies to inhibit HIV-1 replication
- Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells
- DNA constructs designed to produce short hairpin, interfering RNAs in transgenic mice sometimes show early lethality and an interferon response
| Nucleic acids and oligonucleotides edit | ||
|---|---|---|
| Nucleobases: | Adenine | Thymine | Uracil | Guanine | Cytosine | Purine | Pyrimidine | |
| Nucleosides: | Adenosine | Uridine | Guanosine | Cytidine | Deoxyadenosine | Thymidine | Deoxyguanosine | Deoxycytidine | |
| Nucleotides: | AMP | UMP | GMP | CMP | ADP | UDP | GDP | CDP | ATP | UTP | GTP | CTP | cAMP | cADPR | cGMP | |
| Deoxynucleotides: | dAMP | TMP | dGMP | dCMP | dADP | TDP | dGDP | dCDP | dATP | TTP | dGTP | dCTP | |
| Ribonucleic acids: | RNA | mRNA | tRNA | rRNA | ncRNA | sgRNA | shRNA | siRNA | snRNA | miRNA | snoRNA | LNA | |
| Deoxyribonucleic acids: | DNA | mtDNA | cDNA | plasmid | Cosmid | BAC | YAC | HAC | |
| Analogues of nucleic acids: | GNA | PNA | TNA| LNA | morpholino | |
| ←Amino acids | Major families of biochemicals | Carbohydrates→ |
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