Talk:Phosphate buffered saline

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Why is PBS used instead of ordinary saline?


When do you use PBS with calcium and magniesium, and when do you without. When we wash the cells before adding trypsin, we use PBS without magnesium and calcium, but later on we use PBS with calcium and magnesium. why is it so?


  • According to the following page, Ca and Mg promote cell adhesion. Hence, the formulation contained in the article is the one that should be used when detaching cells.

Dulbecco's Media and Salts Selection Guide

I used the think PBS was just one thing, but actually there are lots of recipes, this should be reflected in the article (will add it if I have a chance).

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