Talk:Phage display

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I read that phage can be eluted with trypsin, but if you do that, and it cuts off the displayed protein, then how can you repeat the panning? Coat protein III which is usually what binds the displayed protein to the phage only occurs in one place with upto five units, and rounds of panning are often completed more than five times..? --Username132 16:19, 26 January 2006 (UTC)

KM13 is a popular helper phage that allows for elution using trypsin (although my experience suggests that elution be performed using TEA first, then trypsin treatment). Although this cuts the displayed protein, the genetic information is still contained within the phage. Once you infect bacteria with the eluted phage and amplify, the protein becomes displayed on the coat protein of the amplified phage for subsequent rounds of panning. Hope this helped. 216.199.242.34 17:47, 14 February 2006 (UTC) Warren D. Marcus