Optode

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An opt(r)ode is an optical sensor, that is a device that optically measures a specific substance usually with the aid of a chemical transducer.

An opt(r)ode requires three components to function: a chemical that responds to an analyte, a polymer to immobilise the chemical transducer and instrumentation (optical fibre, light source, detector and other electronics. Opt(r)odes usually have the polymer matrix coated onto the distal tip of an optical fibre, but in the case of evanescent wave opt(r)odes the polymer is coated on a section of fibre that has been unsheathed.

Optrodes can apply various optical measurement schemes such as reflection, absorption, evansescent wave, luminescence (fluorescence and phosphorescences), chemiluminescence, surface plasmon resonance. By far the most popular methodology is luminescence.

Luminescence in solution obeys the Stern-Volmer relationship. This relationship is linear. Fluorescence of a molecule is quenched by specific analytes e.g. Ruthenium complexes are quenched by oxygen. When a fluorophore is immobilised within a polymer matrix a miriad of micro-environments are created. The micro-environments reflect varying diffusion co-efficients for the analyte. This leads to a non-linear relationship between the fluorescence and the quencher (analyte). This relationship is modelled in various ways, the most popular model is the Two site model created by James Demas (University of Virginia)

The signal (fluorescence) to oxygen ratio is not linear, and an optode is most sensitive at low oxygen concentration, i.e. the sensitivity decreases as oxygen concentration increases. The optode sensors can however work in the whole region 0-100% oxygen saturation in water, and the calibration is done the same way as with the Clark type sensor. No oxygen is consumed and hence the sensor is stirring insensitive, but the signal will stabilize more quickly if you stir the sensor after you have put it into your sample.

Optical sensors are growing in popularity due to the low-cost, low power requirements and long term stability. They provide viable alternatives to electrode based sensors or more complicated analytical instrumentation.

Major international conferences are devoted to their development e.g Europtrode VIII Tubeingen 2006, OFS 18, Cancun 2006.


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