Maurice Wilkins

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Maurice Wilkins

Discovery of the DNA Double Helix

Maurice Wilkins
Francis Crick
Rosalind Franklin
James Watson
Maurice Wilkins

Maurice Hugh Frederick Wilkins CBE FRS (15 December 1916 – 5 October 2004) was a New Zealand-born British physicist and Nobel Laureate who contributed research in the fields of phosphorescence, radar, isotope separation, and X-ray diffraction. He was most widely known for his work at King's College London on the structure of DNA, for which he, Francis Crick and James Watson were awarded the 1962 Nobel Prize for Physiology or Medicine for their discoveries concerning the molecular structure of nucleic acids and its significance for information transfer in living material[1]. His KCL colleague Rosalind Franklin made a major contribution to the discovery of the structure of DNA but she had died from cancer in 1958 and was therefore ineligible for the 1962 Nobel Prize award.

Contents

[edit] Early life and career

Wilkins was born in Pongaroa, north Wairarapa, New Zealand where his father was a medical doctor. His family moved to Birmingham, England when he was 6, where he subsequently attended King Edward's School at the age of 11. He later studied physics at St. John's College, Cambridge, then in 1940 he received his Ph.D. in physics at the University of Birmingham with a dissertation on phosphors. During World War II he developed improved radar screens at Birmingham, then worked on isotope separation at the Manhattan Project at the University of California, Berkeley for two years before returning to King's College London. "After the war I wondered what I would do, as I was very disgusted with the dropping of two bombs on civilian centres in Japan," he told Britain's Encounter radio programme in 1999.

In 1946 the physicist John Randall was placed in charge of a new biophysics laboratory at King's College. The plan was to hire physicists such as Wilkins to work on problems in biology. When Francis Crick first met Wilkins he was not convinced that the King's College laboratory had anything like a clear plan of attack. There seemed to be a vague hope that by applying techniques like Ultraviolet light microscopy (Wilkins) and electron microscopy (Randall), new insights could be gained into cell structure and function. By 1950, Randall was gearing up the laboratory for work on proteins. His original plan for Rosalind Franklin was that she do X-ray diffraction studies on proteins. Wilkins' work on DNA changed that. By 1951, Randall had established a major effort to solve the structure of collagen and Wilkins and Franklin represented a parallel effort to determine the structure of DNA. In the meantime, Maurice Wilkins' friend Francis Crick had joined forces with James Watson at the Cavendish Laboratory, Cambridge under Lawrence Bragg.

[edit] DNA
DNA pioneers
William Astbury
Oswald Avery
Erwin Chargaff
Max Delbrück
Jerry Donohue
Raymond Gosling
Phoebus Levene
Linus Pauling
Sir John Randall
Erwin Schrödinger
Alec Stokes
Herbert Wilson

At King's College Wilkins pursued, among other things X-ray diffraction work on DNA that had been obtained from calf thymus by the Swiss scientist Rudolf Signer. The DNA from Signer's lab was much more intact than the DNA which had previously been isolated.[citation needed] Wilkins discovered that it was possible to produce thin threads from this concentrated DNA solution that contained highly ordered arrays of DNA suitable for the production of X-ray diffraction patterns.[2] Using a carefully bundled group of these DNA threads and keeping them hydrated, Wilkins and a graduate student Raymond Gosling obtained X-ray photographs of DNA that showed that the long, thin DNA molecule in the sample from Signer had a regular, crystal-like structure in these threads.[citation needed] This initial X-ray diffraction work at Kings College was done in May or June of 1950. It was one of the X-ray diffraction photographs taken in 1950, shown at a meeting in Naples a year later, that sparked James Watson’s interest in DNA.[citation needed]

At that time Wilkins also introduced Francis Crick to the importance of DNA.[citation needed] Wilkins knew that proper experiments on the threads of purified DNA would require better X-ray equipment. Wilkins ordered a new X-ray tube and a new microcamera. Before the DNA sample from Signer was available, Gosling had been trying to make X-ray diffraction images of sperm.[citation needed] However, Franklin did not start using the new equipment until September 1951.[citation needed] By the summer of 1950 Randall had arranged for a three year research fellowship that would fund Rosalind Franklin in his laboratory. Franklin was delayed in finishing her work in Paris.[citation needed] Late in 1950, Randall wrote to Franklin to inform her that rather than work on protein, she should take advantage of Wilkin's preliminary work and that she should do X-ray studies of DNA fibers made from Signer's DNA.[citation needed] Early in 1951 Franklin finally arrived. Wilkins was away on holiday and missed an initial meeting at which Raymond Gosling stood in for him along with Alexander R. Stokes, who, like Crick, would solve the basic mathematics that make possible a general theory of how helical structures diffract X-rays.[citation needed] No work had been done on DNA in the laboratory for several months; the new X-ray tube sat unused, waiting for Franklin. Franklin ended up with the DNA from Signer, Gosling became her PhD student and she had the expectation that DNA X-ray diffraction work was her project.[citation needed] Wilkins returned to the laboratory expecting that Franklin would be his collaborator and that they would work together on the DNA project that he had started.[citation needed] Franklin felt that DNA was now her project and would not collaborate with Wilkins, who then pursued parallel studies.[citation needed]

By November 1951 Wilkins thought he had evidence that DNA in cells as well as purified DNA had a helical structure[3]. Alexander Stokes had solved the basic mathematics of helical diffraction theory and thought that Wilkin's X-ray diffraction data indicated a helical structure in DNA.[citation needed] Wilkins met with Watson and Crick and told them about his results.[citation needed] This information from Wilkins along with additional information gained by Watson when he heard Franklin talk about her research during a King's College research meeting, stimulated Watson and Crick to create their first molecular model of DNA, a model with the phosphate backbones at the center.[citation needed] Upon viewing the model of the proposed structure, Franklin told Watson and Crick that it was wrong. Franklin knew that because of basic chemical principles the hydrophilic backbones should go on the outside of the molecule where they could interact with water.[citation needed] Crick tried to get Wilkins to continue with additional molecular modeling efforts, but Wilkins did not take this approach. During 1952, Franklin also refused to participate in molecular modeling efforts and continued to work on step-by-step detailed analysis of her X-ray diffraction data (Patterson synthesis).[citation needed] By Spring of 1952, Franklin had received permission from Randall to request permission to transfer her fellowship so that she could leave King's College and work in John Bernal's laboratory.[citation needed] However, Franklin remained at King's College for another year.

By early 1953, it was clear that Franklin would simply drop her DNA work at the end of her fellowship that summer, or even sooner. Linus Pauling had published a proposed but incorrect structure of DNA, making the same basic error that had been made by Watson and Crick a year earlier. Some of those working on DNA in the United Kingdom, feared that Pauling would quickly solve the DNA structure once he recognized his error and put the backbones of the nucleotide chains on the outside of a model of DNA.[citation needed] After March 1952 Franklin concentrated on the X-ray data for the A-form of less hydrated DNA while Wilkins tried to work on the B-form.[citation needed] Wilkins was handicapped because Franklin had all of the good DNA.[citation needed] Wilkins got new DNA, but it was not as good as the original sample he had used in 1950 and which Franklin continued to use.[citation needed] About his only new results were for biological samples like sperm cells, which seemed to also suggest a helical structure for DNA.[citation needed] In the middle of 1952 Wilkins had for a time abandoned further DNA work when Franklin reported to him that her results made her doubt the helical nature of the A-form.[citation needed] Wilkins feared, the data suggesting a helical structure might just be an artifact.[citation needed]

In early 1953 Watson visited King's College and Wilkins showed him a high quality image of the B-form X-ray diffraction pattern that had been produced by Franklin in March 1952.[citation needed] With the knowledge that Pauling was working on DNA and had submitted a model of DNA for publication, Watson and Francis Crick mounted one more concentrated effort to deduce the structure of DNA.[citation needed] Crick gained access to a progress reports from King's College that included useful information from Franklin about the features of DNA she had deduced from her X-ray diffraction data from Max Perutz, his thesis supervisor.[citation needed] Watson and Crick published their proposed DNA double helical structure in a paper in the journal Nature in April of 1953.[citation needed] In this paper Watson and Crick acknowledged that they had been "stimulated by.... the unpublished results and ideas" of Wilkins and Franklin.[citation needed]

In recognition of the contribution from King's College, Watson and Crick agreed that Wilkins, Stokes and Wilson[4] and Franklin and Gosling[citation needed] should each publish their x-ray diffraction work, which supported the proposed Crick-Watson model, in separate articles in the same issue of Nature.[citation needed]

Wilkins and others went on to repeat and extend much of Franklin's work, and produced much evidence to support the helical model of Crick and Watson.

Wilkins married his second wife Patricia Ann Chidgey in 1959.[citation needed] They had four children, Sarah, George, Emily and William; he had a son by his previous marriage, to an art student called Ruth in California.[citation needed]

In 1960 he was presented with the American Public Health Association's Albert Lasker Award,[citation needed] and in 1962 he was made a Companion of the British Empire.[citation needed] Also in 1962 he shared the Nobel Prize in Physiology or Medicine with Watson and Crick.

He published his autobiography, "The Third Man of the Double Helix," in 2003, but does not specifically credit Stokes and Wilson as co-authors of their paper in "Nature". Whether this was deliberate on his part or just down to rather poor sub-editing by OUP is not known.

[edit] See also

[edit] References

  1. ^ The Nobel Prize in Physiology or Medicine 1962. Nobel Prize Site for Nobel Prize in Physiology or Medicine 1962.
  2. ^ See Figure 1 of the Nobel lecture by Wilkins. See other examples at the King's College website for DNA structure.
  3. ^ See Chapter 2 of The Eighth Day of Creation: Makers of the Revolution in Biology by Horace Freeland Judson published by Cold Spring Harbor Laboratory Press (1996) ISBN 0-87969-478-5.
  4. ^ "Molecular Structure of Deoxypentose Nucleic Acids" by M. H. F. Wilkins, A.R. Stokes A.R. and H. R. Wilson in Nature (1953) volume 171 pages 738-740. Download the full text in PDF format.
  1. Watson, James D. "The Double Helix: A Personal Account of the Discovery of the Structure of DNA"; The Norton Critical Edition, which was published in 1980, edited by Gunther S. Stent.

[edit] Books featuring Maurice Hugh Frederick Wilkins

  • Robert Olby; "The Path to The Double Helix: Discovery of DNA"; first published in 0ctober 1974 by MacMillan, with foreword by Francis Crick; ISBN 046681173; the definitive DNA textbook, revised in 1994, with a 9 page postscript.
  • Horace Freeland Judson, "The Eighth Day of Creation. Makers of the Revolution in Biology"; Penguin Books 1995, first published by Jonathan Cape, 1977; ISBN 1-357-91086-42.
  • Watson, James D. The Double Helix: A Personal Account of the Discovery of the Structure of DNA; The Norton Critical Edition , which was published in 1980, edited by Gunther S. Stent:ISBN 0-393-01245-X.
  • Chomet, S. (Ed.), D.N.A. Genesis of a Discovery, 1994, Newman- Hemisphere Press, London; NB a few copies are available from Newman-Hemisphere at 101 Swan Court, London SW3 5RY (phone: 07092 060530).
  • Sayre, Anne 1975. Rosalind Franklin and DNA. New York: W.W. Norton and Company. ISBN 0-393-32044-8.
  • Wilkins, Maurice, The Third Man of the Double Helix: The Autobiography of Maurice Wilkins ISBN 0-19-860665-6.
  • Crick, Francis, 1990. What Mad Pursuit: A Personal View of Scientific Discovery (Basic Books reprint edition) ISBN 0-465-09138-5
  • Watson, James D., The Double Helix: A Personal Account of the Discovery of the Structure of DNA, Atheneum, 1980, ISBN 0-689-70602-2 (first published in 1968)
  • Krude, Torsten (Ed.) DNA Changing Science and Society: The Darwin Lectures for 2003 CUP 2003, includes a lecture by Sir Aaron Klug on Rosalind Franklin's involvement in the determination of the structure of DNA.
  • Ridley, Matt; "Francis Crick: Discoverer of the Genetic Code (Eminent Lives)" was first published in June 2006 in the USA and then in the UK September 2006, by HarperCollins Publishers; 192 pp, ISBN 0-06-082333-X; this book is already being shown on Amazon for advance orders pre-publication. [This short book is in the publisher's "Eminent Lives" series.]
  • * "Light Is A Messenger, the life and science of William Lawrence Bragg" by Graeme Hunter, ISBN 0-19-852921-X; Oxford University Press, 2004.
  • "Designs For Life: Molecular Biology After World War II" by Soraya De Chadarevian; CUP 2002, 444 pp; ISBN 0-521-57078-6; it includes James Watson's "well kept open secret" from April 2003!

[edit] External links

    • 2006 NATIONAL DNA DAY 25TH APRIL 2006

see: http://www.genome.gov/18516768 for the Moderated Chat Transcript Archive

But for the 'second' DNA story in the New York Times, see [4] - for reproduction of the original text in June 1953.

DNA structure research at King's College London 1947-1959
Rosalind Franklin | Raymond Gosling | John Randall | Alec Stokes | Maurice Wilkins | Herbert Wilson
Nobel Prize in Physiology or Medicine: Laureates (1951-1975)

1951: Theiler | 1952: Waksman | 1953: Krebs, Lipmann | 1954: Enders, Weller, Robbins | 1955: Theorell | 1956: Cournand, Forssmann, Richards | 1957: Bovet | 1958: Beadle, Tatum, Lederberg | 1959: Ochoa, Kornberg | 1960: Burnet, Medawar | 1961: Békésy | 1962: Crick, Watson, Wilkins | 1963: Eccles, Hodgkin, Huxley | 1964: Bloch, Lynen | 1965: Jacob, Lwoff, Monod | 1966: Rous, Huggins | 1967: Granit, Hartline, Wald | 1968: Holley, Khorana, Nirenberg | 1969: Delbrück, Hershey, Luria | 1970: Katz, Euler, Axelrod | 1971: Sutherland | 1972: Edelman, Porter | 1973: Frisch, Lorenz, Tinbergen | 1974: Claude, Duve, Palade | 1975: Baltimore, Dulbecco, Temin
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