Macrophage
From Wikipedia, the free encyclopedia
Macrophages (Greek: "big eaters", makros = large, phagein = eat) are cells within the tissues that originate from specific white blood cells called monocytes. Monocytes and macrophages are phagocytes, acting in both nonspecific defence (or innate immunity) as well as specific defense (or cell-mediated immunity) of vertebrate animals. Their role is to phagocytize (engulf and then digest) cellular debris and pathogens either as stationary or mobile cells, and to stimulate lymphocytes and other immune cells to respond to the pathogen.
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[edit] Life cycle
Macrophages are differentiated from monocytes, which are eater cells in the blood, themselves derived from the bone marrow. When a monocyte enters the attacked tissue through the endothelium of a blood vessel (a process known as the leukocyte adhesion cascade), it undergoes a series of changes and becomes a macrophage. Macrophages are attracted to a damaged site by chemical substances through chemotaxis, triggered by a range of different stimuli including damaged cells, pathogens, histamine released by mast cells and basophils, and cytokines released by macrophages already at the site.
Unlike short-lived neutrophils — the phagocytes arriving earlier to sites of infection that live just a few days — the life span of a macrophage ranges from months to years. Macrophages are unable to divide and must mature from monocytes produced in the bone marrow.
[edit] Function
a. Ingestion through phagocytosis, a phagosome is formed
b. The fusion of lysosomes with the phagosome creates a phagolysosome; the pathogen is broken down by enzymes
c. Waste material is expelled or assimilated (the latter not pictured)
Parts:
1. Pathogens
2. Phagosome
3. Lysosomes
4. Waste material
5. Cytoplasm
6. Cell membrane
[edit] Phagocytosis
One important main role of macrophage is the removal of necrotic debris and dust in the lungs. Removing dead cell material is important in chronic inflammation as the early stages of inflammation are dominated by neutrophil granulocytes, which are ingested by macrophages if they come of age (see CD-31 for a description of this process.)
The removal of dust and necrotic tissue is to a greater extent handled by fixed macrophages, which will stay at strategic locations such as the lungs, liver, neural tissue, bone, spleen and connective tissue, ingesting foreign materials such as dust and pathogens, calling upon wandering macrophages if needed.
When a macrophage ingests a pathogen, the pathogen becomes trapped in a food vacuole, which then fuses with a lysosome. Within the lysosome, enzymes and toxic peroxides digest the invader. However, some bacteria, such as Mycobacterium tuberculosis, have become resistant to these methods of digestion. Macrophages can digest more than 100 bacteria before they finally die due to their own digestive compounds.
Macrophages are involved in cell-mediated immunity along with NK cells and cytotoxic T cells.
[edit] Role in specific immunity
After digesting a pathogen, a macrophage will present the antigen (a molecule, most often a protein found on the surface of the pathogen, used by the immune system for identification) of the pathogen to a corresponding helper T cell. The presentation is done by integrating it into the cell membrane and displaying it attached to a MHC class II molecule, indicating to other white blood cells that the macrophage is not a pathogen, despite having antigens on its surface.
Eventually the antigen presentation results in the production of antibodies that attach to the antigens of pathogens, making them easier for macrophages to adhere to with their cell membrane and phagocytize. In some cases, pathogens are very resistant to adhesion by the macrophages. Coating an antigen with antibodies could be compared to coating something with Velcro to make it stick to fuzzy surfaces.
The antigen presentation on the surface of infected macrophages (in the context of MHC class II) in a lymph node stimulates TH1 (type 1 helper t-cells) to proliferate (mainly due to IL-12 secretion from the macrophage). When a B-cell in the lymph node recognizes the same unprocessed surface antigen on the bacterium with its surface bound antibody, the antigen is endocytosed and processed. The processed antigen is then presented in MHCII on the surface of the B-cell. TH1 receptor that has proliferated recognizes the antigen-MHCII complex (with co-stimulatory factors- CD40 and CD40L) and causes the B-cell to produce antibodies that help opsonization of the antigen so that the bacteria can be better cleared by phagocytes.
[edit] Involvement in symptoms of diseases
Due to their role in phagocytosis, macrophages are involved in many diseases of the immune system. For example, they participate in the formation of granulomas, inflammatory lesions that may be caused by a large number of diseases.
Some disorders, mostly rare, of ineffective phagocytosis and macrophage function have been described.
Macrophages are the predominant cells involved in creating the progressive plaque lesions of atherosclerosis.
When fighting influenza, macrophages are dispatched to the throat. However, until the killer T cells for the flu virus are found, the macrophages do more damage than help. They not only destroy throat cells infected with the flu virus but also destroy several surrounding non-infected cells.
Macrophages also play a role in Human Immunodeficiency Virus (HIV) infection. Like T cells, macrophages can be infected with HIV, and even become a reservoir of ongoing virus replication throughout the body.
Macrophages are versatile cells that play many roles. As scavengers, they rid the body of worn-out cells and other debris. Foremost among the cells that "present" crucial role in initiating the immune response. As secretory cells, monocytes and macrophages are vital to the regulation of immune responses and the development of inflammation; they churn out an amazing array of powerful chemical substances (monokines) including enzymes, complement proteins, and regulatory factors such as interleukin-1. At the same time, they carry receptors for lymphokines that allow them to be "activated" into single-minded pursuit of microbes and tumor cells
[edit] Fixed macrophages
As mentioned earlier, a majority of macrophages are stationed at strategic points where microbial invasions or accumulations of dust are likely to occur, each type of macrophage, determined by its location has a specific name:
| Name of cell | Location |
| Alveolar macrophages/dust cells | pulmonary alveolus of lungs |
| Histiocytes | connective tissue |
| Kupffer cells | liver |
| Microglial cells | neural tissue |
| Osteoclasts | bone |
| Sinusoidal lining cells | spleen |
Together this demonstrates that it is highly relevant to know more concerning the function and role of this particular liver cell population
Investigations concerning Kupffer cells are hampered, because in humans Kupffer cells are only accessible for immunohistochemical analysis, from biopsies or autopsies. From rats and mice they are difficult to isolate and after purification only approximately 5 million cells can be obtained from one mouse.
[edit] Related cells
- Tingible body macrophages are found in the germinal centers of lymph nodes
- Dendritic cells (including Langerhans cells)
- A lipid-laden macrophage is called a "foam cell"
