IPTG
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IPTG is a common abbreviation for Isopropyl β-D-1-thiogalactopyranoside, a molecular biology reagent. It has a molecular weight of 238.3.
This compound is used as a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon. Unlike allolactose, the sulfur (S) atom creates a chemical bond which is non-hydrolyzable by the cell, preventing the cell from "eating up" or degrading the inductant.
IPTG induces activity of beta-galactosidase, an enzyme that promotes lactose utilization, by binding and inhibiting the lac repressor. In cloning experiments, the lacZ gene is replaced by your gene of interest and IPTG is then used to induce gene expression.
Many regulatory elements of the lac operon are used in inducible recombinant protein systems; IPTG is an effective inducer in the concentration range of 100 μM to 1.5 mM.
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