Hot 100 number-one hits of 2000 (USA)
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This is a list of the U.S. Billboard magazine Hot 100 number-ones of 2000.
| Issue Date | Song | Artist |
| January 1 | Smooth | Santana featuring Rob Thomas |
| January 8 | Smooth | Santana featuring Rob Thomas |
| January 15 | What a Girl Wants | Christina Aguilera |
| January 22 | What a Girl Wants | Christina Aguilera |
| January 29 | I Knew I Loved You | Savage Garden |
| February 5 | I Knew I Loved You | Savage Garden |
| February 12 | I Knew I Loved You | Savage Garden |
| February 19 | Thank God I Found You | Mariah Carey featuring Joe and 98 Degrees |
| February 26 | I Knew I Loved You | Savage Garden |
| March 4 | Amazed | Lonestar |
| March 11 | Amazed | Lonestar |
| March 18 | Say My Name | Destiny's Child |
| March 25 | Say My Name | Destiny's Child |
| April 1 | Say My Name | Destiny's Child |
| April 8 | Maria Maria | Santana featuring The Product G&B |
| April 15 | Maria Maria | Santana featuring The Product G&B |
| April 22 | Maria Maria | Santana featuring The Product G&B |
| April 29 | Maria Maria | Santana featuring The Product G&B |
| May 6 | Maria Maria | Santana featuring The Product G&B |
| May 13 | Maria Maria | Santana featuring The Product G&B |
| May 20 | Maria Maria | Santana featuring The Product G&B |
| May 27 | Maria Maria | Santana featuring The Product G&B |
| June 3 | Maria Maria | Santana featuring The Product G&B |
| June 10 | Maria Maria | Santana featuring The Product G&B |
| June 17 | Try Again | Aaliyah |
| June 24 | Be with You | Enrique Iglesias |
| July 1 | Be with You | Enrique Iglesias |
| July 8 | Be with You | Enrique Iglesias |
| July 15 | Everything You Want | Vertical Horizon |
| July 22 | Bent | matchbox twenty |
| July 29 | It's Gonna Be Me | 'N Sync |
| August 5 | It's Gonna Be Me | 'N Sync |
| August 12 | Incomplete | Sisqó |
| August 19 | Incomplete | Sisqó |
| August 26 | Doesn't Really Matter | Janet |
| September 2 | Doesn't Really Matter | Janet |
| September 9 | Doesn't Really Matter | Janet |
| September 16 | Music | Madonna |
| September 23 | Music | Madonna |
| September 30 | Music | Madonna |
| October 7 | Music | Madonna |
| October 14 | Come on Over Baby (All I Want Is You) | Christina Aguilera |
| October 21 | Come on Over Baby (All I Want Is You) | Christina Aguilera |
| October 28 | Come on Over Baby (All I Want Is You) | Christina Aguilera |
| November 4 | Come on Over Baby (All I Want Is You) | Christina Aguilera |
| November 11 | With Arms Wide Open | Creed |
| November 18 | Independent Women Part I | Destiny's Child |
| November 25 | Independent Women Part I | Destiny's Child |
| December 2 | Independent Women Part I | Destiny's Child |
| December 9 | Independent Women Part I | Destiny's Child |
| December 16 | Independent Women Part I | Destiny's Child |
| December 23 | Independent Women Part I | Destiny's Child |
| December 30 | Independent Women Part I | Destiny's Child |
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