DNA gyrase

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The current mechanochemical model of Gyrase activity.

DNA gyrase, often referred to simply as gyrase, is a type II topoisomerase (EC 5.99.1.3) that introduces negative supercoils (or relaxes positive supercoils) into DNA by looping the template so as to form a crossing, then cutting one of the double helices and passing the other through it before resealing the break, changing the linking number by two in each enzymatic step.

The unique ability of gyrase to introduce negative supercoils into DNA is what allows bacterial DNA to have free negative supercoils. The ability of gyrase to relax positive supercoils comes into play during DNA replication. The right-handed nature of the DNA double helix causes positive supercoils to accumulate ahead of a translocating enzyme, in the case of DNA replication, a DNA polymerase. The ability of gyrase (and topoisomerase IV) to relax positive supercoils allows superhelical tension ahead of the polymerase to be released so that replication can continue.

1 Mechanochemical Model of Gyrase Activity
2 Inhibition by Antibiotics
3 See also
4 References

[edit] Mechanochemical Model of Gyrase Activity

A single molecule study^[1] that has characterized gyrase activity as a function of DNA tension (applied force) and [ATP] has proposed the mechanochemical model shown in the figure. Upon binding to DNA (the "Gyrase-DNA" state), there is a competition between DNA wrapping and dissociation, where increasing DNA tension increases the probability of dissociation. Upon wrapping and ATP hydrolysis, two negative supercoils are introduced into the template, providing opportunities for subsequent wrapping and supercoiling events.

[edit] Inhibition by Antibiotics

Gyrase is found in bacteria and plants, but not in humans. This makes gyrase a good target for antibiotics. Two classes of antibiotics that inhibit gyrase are the coumarins (including novobiocin), and the quinolones (including nalidixic acid and ciprofloxacin).

DNA gyrase is a member of a class of enzymes called topoisomerases. Quinolones bind these enzymes and prevent them from decatenating replicating DNA. Quinolone resistant bacteria frequently harbor mutated topoisomerases which resist quinolone binding.