Bimolecular fluorescence complementation
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Bimolecular fluorescence complementation (BiFC) is a method of viewing the association of proteins inside living cells. Kerppola et al. (2002) solidified this methodology as viable in vivo.
[edit] How it works
Two non-fluorescent fragments of a fluorescent protein are fused (see fused protein) to proteins of interest. When the two proteins of interest associate, the fragments join and fluoresce. This fluorescence is detected and recorded via a camera mounted on a fluorescence microscope.
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