Alexa (fluor)

From Wikipedia, the free encyclopedia

You have new messages (last change).
  MW (amu) Absorb (nm) Emit (nm) ε (cm-1M-1)
Alexa Fluor 350 410 346 442 19,000
Alexa Fluor 405 1028 401 421 34,000
Alexa Fluor 430 702 434 541 16,000
Alexa Fluor 488 643 495 519 71,000
Alexa Fluor 500 700 502 525 71,000
Alexa Fluor 514 714 517 542 80,000
Alexa Fluor 532 721 532 554 81,000
Alexa Fluor 546 1079 556 573 104,000
Alexa Fluor 555 ~1250 555 565 150,000
Alexa Fluor 568 792 578 603 91,300
Alexa Fluor 594 820 590 617 90,000
Alexa Fluor 610 1172 612 628 138,000
Alexa Fluor 633 ~1200 632 647 100,000
Alexa Fluor 647 ~1300 650 665 239,000
Alexa Fluor 660 ~1100 663 690 132,000
Alexa Fluor 680 ~1150 679 702 184,000
Alexa Fluor 700 ~1400 702 723 192,000
Alexa Fluor 750 ~1300 749 775 240,000

The Alexa Fluor family of fluorescent dyes is produced by Molecular Probes, a subsidiary of Invitrogen. Alexa Fluor dyes are typically used as cell and tissue labels in cell biology.

The excitation and emission spectra of the Alexa Fluor series cover the full range of the visible spectrum and extend into the infrared. The members of the family are numbered according roughly to their excitation maxima (in nm).

Alexa Fluor dyes are synthesized through sulfonation of coumarin, rhodamine, xanthene (such as fluorescein), and cyanine dyes. Sulfonation makes Alexa Fluor dyes negatively charged and hydrophilic.

Alexa Fluor dyes are generally more stable, brighter, and less pH-sensitive than other standard dyes of comparable excitation and emission. However, they tend to be more expensive.

Alternatives to Alexa Fluor dyes include Hilyte fluor dyes manufactured by Anaspec.

[edit] History

The Alexa Fluor dyes were named after Alex Haugland, son of the founders of Molecular Probes, Richard and Rosaria Haugland. The Marina Blue dye was named after their daughter, Marina.

[edit] External links

Retrieved from "http://en.wikipedia.org../../../a/l/e/Alexa_%28fluor%29.html"