Acridine orange

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Acridine orange
General
Systematic name	N,N,N',N'-tetramethylacridine- 3,6-diamine
Other names	Euchrysine 3,6-Acridinediamine Waxoline Orange A Acridine Orange Base Solvent Orange 15 Rhoduline Orange Rhoduline Orange N Acridine Orange NO Rhoduline Orange NO Basic Orange 14
Molecular formula	C17H19N3
SMILES	CN(C)C1=CC2=C(C=C1)C= C3C=CC(=CC3=N2)N(C)C
Molar mass	265.35 g/mol
Appearance	Orange powder
CAS number	[10127-02-3]
Hazards
MSDS	External MSDS
EU classification	Xi N
NFPA 704	0 2 0
R-phrases	R20/21/22 R40
S-phrases	S26 S28 S37 S45
RTECS number	AR7601000
Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) Infobox disclaimer and references

Acridine Orange is a nucleic acid selective fluorescent cationic dye useful for cell cycle determination. It is cell-permeable, and interacts with DNA and RNA by intercalation or electrostatic attractions. When bound to DNA, it is very similar spectrally to fluorescein, with an excitation maximum at 502 nm and an emission maximum at 525 nm (green). When it associates with RNA, the excitation maximum shifts to 460 nm (blue) and the emission maximum shifts to 650 nm (red). The dye is often used in epifluorescence microscopy.

Acridine orange is prepared from coal tar and creosote oil.

[edit] External links

• Acridine orange at PubChem

• Links to external chemical sources.