4Pi STED microscopy
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The 4Pi-STED-microscope is the result of combining the two unrelated concepts of STED- and 4Pi-microscopy. Here, the fluorescent sample is placed in the common focus of two opposing lenses, but excitation and detection are performed through a single lens. The green excitation pulse is immediately followed by a red STED-pulse, which enters the focal region through both lenses inducing stimulated emission of the excited fluorescent molecules to the ground state.
